Isolation, Identification and Nutritional Optimization of Phytase Producers from North Gujarat using Ovat Approach

Abstract

Soil samples collected from twelve distinct locations across the North Gujarat region were systematically screened 
to isolate efficient phytase-producing bacteria. A total of 42 isolates exhibiting positive phytate hydrolysis were obtained 
and characterized based on their morphological features. Among these, three isolates DSS1, CDS1 and BGS3 
demonstrated superior hydrolytic capabilities and were therefore selected for detailed nutritional optimization using a 
one-variable-at-a-time (OVAT) approach. Nutritional optimization revealed isolate-specific preferences for substrate 
components. Wheat bran and yeast extract supported the highest phytase yields in DSS1 and CDS1, whereas rice bran 
combined with ammonium sulphate was optimal for BGS3. Metal ion supplementation further enhanced enzyme 
production, with Ca²⁺ significantly improving phytase activity in DSS1 and BGS3, while Mg²⁺ served as the most 
effective enhancer for CDS1. Overall, this study highlights the diversity of indigenous phytase-producing bacteria in 
North Gujarat soils and underscores the importance of tailored nutritional optimization to maximize enzyme production 
for potential biotechnological and feed industry applications.